不同肌肉特异性启动子IGF2表达载体构建及对牛骨骼肌卫星细胞增殖的影响

doi:10.11843/j.issn.0366-6964.2015.04.007

Abstract

Abstract:

The aim of this study was to construct IGF2 expression vector with efficient and specific promoter and study its effects on bovine skeletal muscle satellite cell proliferation.The 3 eukaryotic expression vectors containing IGF2 and muscle-specific promoter were transfected into bovine skeletal muscle satellite cells and fetal fibroblasts，the relative expression of IGF2 was detected by RT-PCR to screen efficient and specific vector.EdU experiments were carried out and RT-PCR was used to detect the cell cycle-related genes.The different promoter （desminpro，CMV-MyoGpro and MyoGpro-double） IGF2 expression vectors were constructed successfully.The test results of RT-PCR showed that pGL3-desminpro-IGF2 was an efficient and specific vector.The vector transfected into cultured cell in vitro increased the speed of cell proliferation and up-regulated the expression of cell cycle-related genes，CDK6 and IGF2.The result has laid an important foundation for the production of transgenic bovine.

CLC Number:

S823
S813.3

SUN Xiao-li，LI Shu-feng，TONG Hui-li，ZHANG Wei-wei，YIN Hong-yan，YAN Yun-qin. The Construction of Different Muscle-specific Promoter IGF2 Expression Vector and Its Impact on Bovine Skeletal Muscle Satellite Cell Proliferation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 555-560.

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The Construction of Different Muscle-specific Promoter IGF2 Expression Vector and Its Impact on Bovine Skeletal Muscle Satellite Cell Proliferation

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